Secreted frizzled-related protein 5 (SFRP5) protects ATDC5 cells against LPS-induced inflammation and apoptosis via inhibiting Wnt5a/JNK pathway

Abstract Background Secreted frizzled-related protein 5 (SFRP5) is an endogenous inhibitor of Wnt5a (wingless-type family member 5a), which has been implicated in anti-inflammatory response. In this study, we aimed to investigate whether SFRP5 could protect chondrocytes against LPS-induced inflammation and apoptosis. Methods ATDC5 cells that overexpressed with or not were challenged LPS observe the effects overexpression on LPS-triggered apoptosis as well Wnt5a/JNK activation. was elevated presence overexpression, determine signaling involved actions SFRP5. Results The mRNA levels significantly reduced by a concentration-dependent manner. Overexpression inhibited apoptosis, evidenced decreased production TNF-?, IL-1?, IL-6, ROS, together ratio TUNEL-positive cells, lower expression Bax cleaved caspase 3, but higher Bcl-2. Meanwhile, also repress phosphorylated JNK expression. However, considerably weakened inhibitory effect Besides, level phosphorylation, partially recovered overexpression. Conclusion alleviate cell apoptosis; these may rely repressing